Voltage-gated calcium channels

Calcium Channel Screening Assays

Voltage-gated calcium channels

Voltage-gated calcium channels (VGCC) are found in excitable cells such as muscle and neurons and consist of an α1 ion-conducting pore which forms a complex with associated subunits (α2δ, β1-4, γ). There are various voltage-gated calcium channel α1 subtypes, namely the L-type (Cav1.1, 1.2, 1.3, 1.4), P/Q-type (Cav2.1), N-type (Cav2.2), R-type (Cav2.3) and T-type (Cav3.1, 3.2. 3.3) channels.

SB Drug Discovery has developed stable calcium channel cell lines and validated screening & profiling assays for HTS, lead optimization studies and selectivity profiling. Our assays have been validated by experienced in-house electrophysiologists using rigorously optimized SOPs to ensure data of the highest quality and consistency.


 Fluorescence Assays

Calcium channel assay
Calcium channel assay

Calcium channel assays: Optimized screening assays for voltage-gated calcium channels. SB offers single concentration screening and concentration-response studies against a range of voltage-gated calcium channels using 96 & 384-well FLIPR platforms.


 Calcium Channel Electrophysiology

384-well automated electrophysiology offers the flexibility to run single concentration, high-throughput calcium channel screening as well as detailed profiling studies using standard and customized protocols. SB offers electrophysiology assays for L, P/Q, N and T-type calcium channels designed around your specific requirments, 

Calcium channel assay
Calcium channel assay

Calcium channel electrophysiology: Cav1.2 electrophysiology assays available for HTS and cardiac safety studies using state-of-the-art 384-well electrophysiology.

 

Cav1.2 screening assay
Cav1.2 screening assay

Control time courses: Optimized high throughput screening assays for cardiac Cav1.2 stable cell line. Left panel shows DMSO control (blue) followed by full block (grey), right panel shows 1 μM nifedipine (blue) followed by full block (grey).

 

Cav2.1 screening assay
Cav2.1 screening assay

State-dependency assays: Assessment of state-dependency using 2-pulse protocols. Example shows Cav2.1 DMSO control (left) and reference compound (right).


Calcium Channel Assays

Cav1.2 (β2/α2δ) Cav1.2 (β3/α2δ) Cav1.3 (β3/α2δ) Cav1.4 (β3/α2δ)
Cav2.1 (β3/α2δ) Cav2.2 (β3/α2δ) Cav3.1 Cav3.2

To discuss your next ion channel project or to receive a quote, visit our contact page or call +44 141 587 6100.


Ion channel cell lines
Ion channel screening