Cav1.2 assay

Cav1.2 Screening Assays

Species: Human

Subunits / Accessory Proteins: β2, α2δ1

Cell Type: HEK-293

Assay Platforms: Fluorescence, Manual Patch Clamp, Automated Electrophysiology

Services: HTS, single concentration / dose response studies, selectivity profiling

Cav1.2 - Manual Patch Clamp

Cav1.2 assay

Figure 1: Concentration-dependent effects of FPL-61476 on ICa currents (pA) obtained from HEK-293 cells stably transfected with human Cav 1.2 channels. Calculated EC50 value 4 µM.

Cav1.2 - Manual Patch Clamp

Cav1.2 assay

Figure 2: Concentration-dependent effect of nifedipine on ICa currents (pA) obtained from HEK-293 cells stably transfected with human Cav 1.2 channels. Calculated IC50 value 49 nM.

Cav1.2 - SyncroPatch

Cav1.2 assay

Figure 3: Superimposed current traces during application of a single concentration (100nM) of reference compound. Control current is shown in black.

Cav1.2 - SyncroPatch

Cav1.2 assay

Figure 4: Time-course of effect produced by 100nM Nifedipine (blue area). The darker grey column shows maximum concentration of reference blocker.


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