Inflammation

Cytokine Profiling

In-depth assessment of inflammatory responses.

SB's cytokine profiling assays are designed to measure cytokines and chemokines released from chosen target cells. These assays can be customized to detect specific groups of cytokines of interest, using either real-time PCR or ELISA platforms.

Customize your assays

Starting Material: Whole blood / neutrophils / PBMC / leukocyte subtypes / epithelial cells / fibroblasts

Stimulants: LPS / fMLP / PMA / IL-17 / poly I:C / IL-8 / anti-CD3/CD28

Cytokines available

TNFα IL-1β IL-2 IL-4 IL-5 IL-6
IL-7 IL-8 IL-9 IL-10 IL-12 (p70) IL-13
IL-15 IL-17a FGF Eotaxin G-CSF GM-CSF
IFNγ IP-10 MCP-1 MIP-1α MIP-1β PDGF-BB
RANTES VEGF        

Examples:

IL-6

cytokine profiling

Figure 1: IL-6 measurements following stimulation of human primary bronchial epithelial cells with IL-17.

IL-8

cytokine profiling

Figure 2: IL-8 measurements following stimulation of human primary bronchial epithelial cells with IL-17.

 

IL-1β

cytokine profiling

Figure 3: IL-1β measurements following stimulation of fresh human PBMCs with LPS, LPS + GM-CSF & LPS + IFN. 

 

Gene expression profiling

cytokine profiling

Figure 4: Cytokine gene expression profiling following stimulation of fresh human PBMCs with LPS.


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