Inflammation

Functional Cell-Based Inflammatory Assays

Customized assays for inflammatory drug discovery.

SB's inflammation team has expertise in running a broad range of functional assays using primary cells and immortalized cell lines. A range of assay services are available off the shelf as well as customizable assays tailored to your choice of cell model and assay readout.

 

Cyclic Nucleotide Signalling

Request a quote

cAMP a549 assay

Figure 1: Dose response graph of reference compound in an A549 PDE4 cell based assay. Cells were incubated with compound and stimulated with Forskolin before measuring cAMP levels. Reference IC50 value ~ 2.4 µM.

 

cGMP PDE5 assay

Figure 2: Dose response graph of reference compound in an HEK-PDE5 cell based assay. Cells were incubated with compound and stimulated with SNP before measuring cGMP levels by ELISA. Reference IC50 value ~ 0.7 µM.

 

Superoxide Release (Respiratory Burst)

Request a quote

Superoxide Release Assay

Figure 3: Concentration-dependent effect of reference inhibitors on respiratory burst output from human granulocytes stimulated with fMLP.

 

Superoxide Release Assay

Figure 4: Concentration-dependent effect of reference inhibitors on respiratory burst output from human granulocytes stimulated with fMLP.

 

Neutrophil Elastase Release

Request a quote

neutrophil elastase release assay

Figure 5: Effect of fMLP on elastase release in human neutrophils, measured as rate of substrate cleavage.

 


Back to Overview
Request a Screening Quote