GPCR assays

Muscarinic (Acetylcholine) Receptor Assays

Species: Human

Subtypes: M1, M2, M3, M4, M5

Assay Formats: Radioligand binding (M1, M2, M3, M4, M5), Cell-based assay (M1, M2, M3, M4, M5)

Services: HTS, single concentration / dose response studies, selectivity profiling

 

Muscarinic M1 - Ligand binding assay

Muscarinic M1 binding assay

Figure 1: Dose response curve for Atropine in a Muscarinic M1 radioligand binding assay. Approximate IC50 89nM.

 

Muscarinic M2 - Ligand binding assay

Muscarinic M2 binding assay

Figure 2: Dose response curve for Atropine in a Muscarinic M2 radioligand binding assay. Approximate IC50 266nM.

 

Muscarinic M3 - Ligand binding assay

Muscarinic M3 binding assay

Figure 3: Dose response curve for Atropine in a Muscarinic M3 radioligand binding assay. Approximate IC50 123nM.

 

Muscarinic M4 - Ligand binding assay

Muscarinic M4 binding assay

Figure 4: Dose response curve for Atropine in a Muscarinic M4 radioligand binding assay. Approximate IC50 52nM.

 

Muscarinic M5 - Ligand binding assay

Muscarinic M5 binding assay

Figure 5: Dose response curve for Atropine in a Muscarinic M5 radioligand binding assay. Approximate IC50 50nM.

 

Muscarinic M1 - Cell-based assay

Muscarinic M1 cell based assay

Figure 6: Dose response curve for Atropine in a Muscarinic M1 cell-based assay. Approximate IC50 1.2nM.

 

Muscarinic M2 - Cell-based assay

Muscarinic M1 cell based assay

Figure 7: Dose response curve for Atropine in a Muscarinic M2 cell-based assay. Approximate IC50 0.5nM.

 

Muscarinic M3 - Cell-based assay

Muscarinic M3 cell based assay

Figure 8: Dose response curve for Atropine in a Muscarinic M3 cell-based assay. Approximate IC50 0.75nM.

 

Muscarinic M4 - Cell-based assay

Muscarinic M4 cell based assay

Figure 9: Dose response curve for Atropine in a Muscarinic M4 cell-based assay. Approximate IC50 12nM.

 

Muscarinic M5 - Cell-based assay

Muscarinic M5 cell based assay

Figure 10: Dose response curve for Atropine in a Muscarinic M5 cell-based assay. Approximate IC50 140nM.


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