Ion Channel Assays

HCN4 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: HEK293

Assay Platforms: Fluorescence, Manual Patch Clamp, QPatch

Services: HTS, single concentration / dose response studies, selectivity profiling

HCN4 - QPatch

hcn4 assay

Figure 1: Voltage-dependent activation of HCN4 currents obtained from stably transfected HEK-293 cells (peak current amplitude at -140mV: -2570±690 pA).

HCN4 - QPatch

hcn4 assay

Figure 2: Concentration-dependent effect of antagonist Ivabradine (at -140mV) on HEK-293 cells stably transfected with HCN4 channels.  Calculated IC50 value 30 μM.


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