Inflammation

In vivo Models

Proof of concept inflammatory studies.

Ovalbumin (OVA)-Challenged Mouse Model of Allergic Asthma

The ovalbumin-sensitized mouse model remains the Gold-standard rodent model for allergic asthma. Mice exposed to an OVA-challenge develop pulmonary inflammation, characterized by a marked increase in eosinophil numbers. This, together with hyper-responsiveness (AHR) to spasmogens, is a characteristic feature of asthma in humans. AHR is assessed in this model using barometric whole body plethysmograph (Buxco system) in unrestrained and conscious mice. Inflammatory parameters such as cytokine measurements, differential cell counts and protein content of the bronchoalveolar lavage fluid are routinely examined.

LPS Mouse Model of Chronic Obstructive Pulmonary Disease (COPD)

This model is based on the model described by Vernooy et al (Am J Res Cell Mol Biol (2002) 26: 152-159). Mice are challenged twice per week with a low dose of LPS over a 10 week period before being administered study compound. Inflammatory parameters such as cytokine measurements, differential cell counts and protein content of the bronchoalveolar lavage fluid are routinely studied.

LPS Model of Acute Lung Injury

Intra-tracheal instillation of lipopolysaccharide (LPS) results in an acute, Th1-type inflammatory response in these mice. Cytokines such as TNF-a and CXC chemokines such as MIP-2 and KC are involved in the trafficking of neutrophils from the circulation into the alveolar spaces. Inflammatory parameters such as cytokine measurements, differential cell counts and protein content of the bronchoalveolar lavage fluid are routinely examined. Pulmonary edema, as measured by an increase in lung water content, occurs following this inflammatory response.

To discuss your specific requirements contact us.


Back to Overview
Request a Screening Quote