TRP Channels

Mouse TRPM5 Screening Assays

Species: Mouse

Subunits / Accessory Proteins: N/A

Cell Type: CHO

Assay Platforms: Fluorescence, Manual Patch Clamp, QPatch

Services: HTS, single concentration / dose response studies, selectivity profiling

Mouse TRPM5 - QPatch Assay

trpm5 assay

Figure 1: Example of a plotted time course of effect of free internal Ca2+ (10 µM) in combination with increasing concentrations of ionomycin on mouse TRPM5 currents. A pH of 5.0 was used to block mouse TRPM5 currents.

Mouse TRPM5 - QPatch Assay

trpm5 assay

Figure 2: Normalised response of externally applied ionomycin in combination with internal free Ca2+ obtained from CHO cells stably expressing mouse TRPM5 channels. The calculated ionomycin EC50 was approximately 3 µM .


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