Ion Channel Assays

nAChR α3β4 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: HEK293

Assay Platforms: Fluorescence, Automated Electrophysiology

Services: HTS, single concentration / dose response studies, selectivity profiling

nAChR α3β4 - Fluorescence Assay

nicotinic nachr alpha3beta4 assay

Figure 1:  Concentration dependent effect of agonist Nicotine on HEK-293 cells stably transfected with nicotinic α3β4 receptor. Calculated EC50 approximately  692 nM.

nAChR α3β4 - Fluorescence Assay

nicotinic nachr alpha3beta4 assay

Figure 2:  Concentration dependent effect of antagonist SR16584 on HEK-293 cells stably transfected with nicotinic α3β4 receptor. Calculated IC50  approximately 2 μM.    


Back to Family
Request a Screening Quote