Ion Channel Assays

nAChR α7 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: HEK293

Assay Platforms: Fluorescence, Manual Patch Clamp, Automated Electrophysiology

Services: HTS, single concentration / dose response studies, selectivity profiling

nAChR α7 - Fluorescence Assay

nicotinic nachr alpha7 assay

Figure 1:  Concentration dependent effect of agonist on HEK-293 cells stably transfected with nicotinic α7 receptor. Calculated EC50 approximately  0.5µM.

nAChR α7 - Fluorescence Assay

nicotinic nachr alpha7 assay

Figure 2:  Concentration dependent effect of antagonist MLA on HEK-293 cells stably transfected with nicotinic α7 receptor. Calculated IC50  approximately 2 nM.                        

                        

nAChR α7 - Manual Patch Clamp

nicotinic nachr alpha7 assay

Figure 3:  Concentration dependent effect of agonist acetylcholine (+PNU) on HEK-293 cells stably transfected with nicotinic α7 receptor. Calculated EC50 approximately  1.5µM.

nAChR α7- Manual Patch Clamp

nicotinic nachr alpha7 assay

Figure 4: Concentration dependent effect of antagonist methyllcaconitine on HEK-293 cells stably transfected with nicotinic α7 receptor and activated with acetylcholine (+PNU). Calculated IC50 value approximately 2.5nM.


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