Nav1.5 Screening Assays
Subunits / Accessory Proteins: Beta 1
Cell Type: HEK-293
Assay Platforms: Fluorescence, Manual Patch Clamp, Automated Electrophysiology
Services: HTS, single concentration / dose response studies, selectivity profiling
Figure 1: Nav1.5 time-course experiment. Plotted graph expressed as current (nA) versus time (s) showing block of current induced by increasing concentrations of TTX.
Figure 2: Concentration-dependent effect of TTX on INa currents (pA) obtained from cells stably transfected with human NaV1.5 channels. IC50 value 4.8 μM.
Figure 3: superimposed Nav1.5 peak current traces under control conditions (black line) and in the presence of increasing concentrations (0.001-100 µM) of Tetrodotoxin (blue lines).
Figure 4: Time-course showing stability of Nav1.5 peak current amplitude under control conditions, in the presence of increasing concentrations of DMSO and after addition of a saturating concentration of 100 µM Tetrodotoxin.