Inflammation

Cytokine Profiling - Primary Cell Assays

Customized assays for inflammatory drug discovery.

SB's inflammation team has expertise in running a broad range of cytokine profiling assays using primary cells and immortalized cell lines. A range of assay services are available off the shelf as well as customizable assays tailored to your choice of cell model and assay readout.

 

GI Epithelial Cells - Cytokine Profiling

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IL6 intestinal epithelial cells

Figure 1: Effect of Dex on IL-6 release from human intestinal epithelial cells stimulated with LPS.

 

IL8 intestinal epithelial cells

Figure 2: Effect of Dex on IL-8 release from human intestinal epithelial cells stimulated with LPS.

 

Keratinocytes - Cytokine Profiling

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Keratinocyte assay TSLP

Figure 3: TSLP release from human keratinocytes following stimulation with cytokine cocktail (IL-4, IL-13 and TNF alpha) alone or cytokine cocktail supplemented with poly I:C.

Keratinocyte assay TSLP

Figure 4: Effect of Dex on TSLP release from human epidermal keratinocytes. Cells were stimulated with cytokine cocktail supplemented with poly I:C.

Keratinocyte assay IL8

Figure 5: IL-8 release from human keratinocytes following stimulation with cytokine cocktail (IL-4, IL-13 and TNF alpha)  supplemented with poly I:C.

 

Keratinocyte assay IL8

Figure 6: Effect of Dex on IL-8 release from human epidermal keratinocytes. Cells were stimulated with cytokine cocktail supplemented with poly I:C.

 

Bronchial Epithelial Cells - Cytokine Profiling

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Bronchial Epithelial cells IL6

Figure 7: IL-6 release from human bronchial epithelial cells following stimulation with IL-17.

Bronchial Epithelial cells IL6

Figure 8: Effect of SB202190 on IL-6 release from human bronchial epithelial cells stimulated with IL-17.

Bronchial Epithelial cells IL8

Figure 9: IL-8 release from human bronchial epithelial cells following stimulation with IL-17.

 

Bronchial Epithelial cells IL8

Figure 10: Effect of SB202190 on IL-8 release from human bronchial epithelial cells stimulated with IL-17.

 

Fibroblast-like Synoviocytes - Cytokine Profiling

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Synoviocytes IL6

Figure 11: IL-6 release from human fibroblast-like synoviocytes following stimulation with LPS.

Synoviocytes IL6

Figure 12: Effect of SB202190 on IL-6 release from human fibroblast-like synoviocytes stimulated with LPS.

Synoviocytes IL6

Figure 13: IL-6 release from human fibroblast-like synoviocytes following stimulation with IL-17.

 

Synoviocytes IL6

Figure 14: Effect of SB202190 on IL-6 release from human fibroblast-like synoviocytes stimulated with IL-17.

 

Monocyte Cell Line (THP-1) - Cytokine Profiling

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thp1 assay

Figure 15: TNFα release from human monocytic THP-1 cells following stimulation with LPS.

 

thp1 assay

Figure 16: Effect of Dex on TNFα release from human monocytic THP-1 cells following stimulation with LPS.

 

Lung Epithelial Cell Line (A549) - Cytokine Profiling

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A549 IL6

Figure 17: IL-6 release from A549 cells following stimulation with LPS.

 

A549 IL6

Figure 18: Effect of SB202190 on IL-6 release from A549 cells following stimulation with LPS.

 

A549 IL8

Figure 19: IL-8 release from A549 cells following stimulation with LPS.

 

A549 IL8

Figure 20: Effect of SB202190 on IL-8 release from A549 cells following stimulation with LPS.

 


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