Assessment of neutrophil function via respiratory burst assay.
Neutrophil trafficking to the site of tissue damage or pathogen infiltration is one of the earliest events in the cellular immune response. Uncontrolled neutrophil activity, however, can lead to chronic inflammatory conditions, such as COPD.
Neutrophils are extremely active cells and following activation, release a host of proteolytic enzymes (for example, neutrophil elastase) and mediators (for example Leukotriene B4). The measurement of release of superoxide anion, the respiratory burst assay, is a widely used parameter for assessing neutrophil function.
SB's respiratory burst assay is performed using freshly isolated human granulocytes or HL60 cells differentiated by Retinoic Acid treatment. Following stimulation with PMA or fMLP, the release of reactive oxygen species is measured by chemiluminescence.
Figure 1: Concentration-dependent effect of reference inhibitors on respiratory burst output from human granulocytes stimulated with fMLP.
Figure 2: Concentration-dependent effect of reference inhibitors on respiratory burst output from human granulocytes stimulated with fMLP.