TRP Channel Screening Services
Transient receptor potential channels (TRP channels) are a group of more than 30 ion channels and can be sub-divided into 6 families: TRPC (canonical), TRPA (ankyrin), TRPV (vanilloid), TRPM (melastatin), TRPP (polycystic) and TRPML (mucolipin). TRP channels are found throughout the body and mediate a variety of sensations including pain, extreme temperatures, taste and vision and are activated by a broad range of molecules including allicin, capsaicin and menthol.
SB Drug Discovery offers the largest selection of commercially available TRP channel assays, spanning HTS, lead optimization studies and TRP channel selectivity profiling. Our TRP assays have been validated by experienced in-house electrophysiologists using rigorously optimized SOPs to ensure data of the highest quality and consistency.
Comprehensive Assay Portfolio
Validated assays: Robust screening & profiling assays available against 21 human TRP channels as well as an extensive range of species variants.
Customized panels: Profile your compounds against one of our standard TRP panels or design your own custom panel as required.
High Throughput Screening
TRP channel screening assays: Screen compound libraries against any TRP channel of choice using 384-well fluorescence or automated electrophysiology platforms.
Ortholog studies: Assess compound potency on species orthologs using our broad range of animal variants. New species assays can also be custom designed.
SB utilizes Nanion's SyncroPatch 384PE for high throughput automated electrophysiology screening assays. HTS assays can be developed for any of our TRP channel targets and customized to meet your needs. Example below shows TRPC5 screening assay.
High throughput screening: Automated electrophysiology enables high throughput screening of TRP channels using flexible protocol design and generating high quality data.
Stable recordings: Left - Activation of TRPC5 followed by application of test compound (blue) and reference inhibitor (grey). Right - IC50 of reference TRPC5 antagonist.
Automated electrophysiology and manual patch clamp are used for TRP channel profiling assays to determine compound potency and target selectivity. Routine TRP channel profiling using electrophysiology platforms generates high quality data which is critical for medicinal chemistry campaigns.
TRPA1 profiling: Automated electrophysiology enables compound profiling against even the most challenging TRP channel targets. Example shows human TRPA1 profiling assay.
Stable recordings: Left - Activation of TRPA1 followed by application of vehicle (blue) and reference inhibitor (grey). Right - IC50 of reference TRPA1 antagonist.
TRP Channel Assays
|TRPA1||TRPA1 (mouse)||TRPA1 (rat)||TRPA1 (guinea pig)|
|TRPA1 (dog)||TRPA1 (sheep)||TRPA1 (monkey)|
|TRPC5 (mouse)||TRPC5 (rat)||TRPC6||TRPC6 (mouse)|
|TRPV1||TRPV1 (mouse)||TRPV1 (rat)||TRPV2|
|TRPV2 (mouse)||TRPV2 (rat)||TRPV3||TRPV3 (mouse)|
|TRPV3 (rat)||TRPV4||TRPV4 (mouse)||TRPV4 (rat)|
|TRPV5||TRPV5 (mouse)||TRPV5 (rat)||TRPV6|
|TRPV6 (mouse)||TRPV6 (rat)|
To discuss your next ion channel project or to receive a quote, contact us or call +44 141 587 6100.