TRP channel screening

TRP Channel Screening Services

TRP channel screening

Transient receptor potential channels (TRP channels) are a group of more than 30 ion channels and can be sub-divided into 6 families: TRPC (canonical), TRPA (ankyrin), TRPV (vanilloid), TRPM (melastatin), TRPP (polycystic) and TRPML (mucolipin). TRP channels are found throughout the body and mediate a variety of sensations including pain, extreme temperatures, taste and vision and are activated by a broad range of molecules including allicin, capsaicin and menthol.

SB Drug Discovery offers the largest selection of commercially available TRP channel assays, spanning HTS, lead optimization studies and TRP channel selectivity profiling. Our TRP assays have been validated by experienced in-house electrophysiologists using rigorously optimized SOPs to ensure data of the highest quality and consistency.


Comprehensive Assay Portfolio

TRP channel screening

Validated assays: Robust screening & profiling assays available against 21 human TRP channels as well as an extensive range of species variants.

Selectivity Panels

TRP channel selectivity panel

Customized panels: Profile your compounds against one of our standard TRP panels or design your own custom panel as required.


High Throughput Screening

TRP channel screening
TRP channel screening

TRP channel screening assays: Screen compound libraries against any TRP channel of choice using 384-well fluorescence or automated electrophysiology platforms.


Species Orthologs

TRP channel screening
TRP channel screening

Ortholog studies: Assess compound potency on species orthologs using our broad range of animal variants. New species assays can also be custom designed.


Electrophysiology Screening

SB utilizes Nanion's SyncroPatch 384PE for high throughput automated electrophysiology screening assays. HTS assays can be developed for any of our TRP channel targets and customized to meet your needs. Example below shows TRPC5 screening assay.

TRP channel screening
TRP channel screening

High throughput screening: Automated electrophysiology enables high throughput screening of TRP channels using flexible protocol design and generating high quality data.

TRP channel screening
TRP channel screening

Stable recordings: Left - Activation of TRPC5 followed by application of test compound (blue) and reference inhibitor (grey). Right - IC50 of reference TRPC5 antagonist.


Electrophysiology Profiling

Automated electrophysiology and manual patch clamp are used for TRP channel profiling assays to determine compound potency and target selectivity. Routine TRP channel profiling using electrophysiology platforms generates high quality data which is critical for medicinal chemistry campaigns.

TRP channel screening
TRP channel screening

TRPA1 profiling: Automated electrophysiology enables compound profiling against even the most challenging TRP channel targets. Example shows human TRPA1 profiling assay.

TRP channel screening
TRP channel screening

Stable recordings: Left - Activation of TRPA1 followed by application of vehicle (blue) and reference inhibitor (grey). Right - IC50 of reference TRPA1 antagonist.



TRP Channel Assays

TRPA1 TRPA1 (mouse) TRPA1 (rat) TRPA1 (guinea pig)
TRPA1 (dog) TRPA1 (sheep) TRPA1 (monkey)  
       
TRPC1 TRPC3 TRPC4 TRPC5
TRPC5 (mouse) TRPC5 (rat) TRPC6 TRPC6 (mouse)
TRPC6 (rat) TRPC7    
       
TRPM2 TRPM3 TRPM3 (rat) TRPM4
TRPM5 TRPM5 (mouse) TRPM8  
       
TRPML1 TRPML2 TRPML3  
       
TRPV1 TRPV1 (mouse) TRPV1 (rat) TRPV2
TRPV2 (mouse) TRPV2 (rat) TRPV3 TRPV3 (mouse)
TRPV3 (rat) TRPV4 TRPV4 (mouse) TRPV4 (rat)
TRPV5 TRPV5 (mouse) TRPV5 (rat) TRPV6
TRPV6 (mouse) TRPV6 (rat)    

To discuss your next ion channel project or to receive a quote, contact us or call +44 141 587 6100.


Cell Line Development
TRP channel screening