TRPA1 assay

TRPA1 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: HEK-293

Assay Platforms: Fluorescence, Manual Patch Clamp

Services: HTS, single concentration / dose response studies, selectivity profiling

TRPA1 - Fluorescence Assay

TRPA1 assayFigure 1: Dose response curve of agonist AITC against human TRPA1 using calcium sensitive dye assay.  EC50 value 1.81µM. 

TRPA1 - Fluorescence Assay

TRPA1 assayFigure 2: Dose response curve of antagonist ruthenium red against human TRPA1 using calcium sensitive dye assay.  IC50 value 14.8µM.

 

TRPA1 - Manual Patch Clamp

TRPA1 assayFigure 3: Normalised concentration-response curve of agonist AITC against TRPA1 current. EC50 value 0.24 µM.

 

TRPA1 - Manual Patch Clamp

TRPA1 assayFigure 4: Normalised concentration-response curve of antagonist ruthenium red against TRPA1 current activated by AITC. IC50 value 36 µM.

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