TRP Channels

TRPC7 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: HEK-293

Assay Platforms: FLIPR, Manual Patch Clamp, Automated Patch Clamp

Services: HTS, single concentration / dose response studies, selectivity profiling

TRPC7 - Fluorescence Assay

trpc7 assay

Figure 1: Concentration-dependent activation of TRPC7 channels by carbachol. Calculated EC50 value approximately 14.6 μM.

TRPC7 - Fluorescence Assay

trpc7 assay

Figure 2: Concentration-dependent inhibition of TRPC7 channels by SKF 96365. Calculated IC50 value approximately 1.33 μM.

 

TRPC7 - Manual Patch Clamp

trpc7 assay

Figure 3: Normalised concentration-response activation curve of carbachol against TRPC7 channels. Calculated EC50 value was approximately 3µM.  Co-application with 1mM La3+ fully blocked the carbachol-induced activation of TRPC7 channels.

 

TRPC7 - Manual Patch Clamp

trpc7 assay

Figure 4: Normalised concentration-response activation curve of OAG against TRPC7 channels. Calculated EC50 value was approximately 2µM. Co-application with 1mM La3+ fully blocked the OAG-induced activation of TRPC7 channels.


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