TRP Channels

TRPM2 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: HEK-293

Assay Platforms: FLIPR, Manual Patch Clamp

Services: HTS, single concentration / dose response studies, selectivity profiling

TRPM2 - Fluorescence Assay

TRPM2 assay

Figure 1: Concentration-dependent activation of TRPM2 channels by H2O2. Calculated EC50 value 10.9 mM.

TRPM2 - Fluorescence Assay

TRPM2 assay

Figure 2: Concentration-dependent inhibition of TRPM2 channels by 2-APB. Calculated IC50 value 103 nM.

 

TRPM2 - Manual Patch Clamp

TRPM2 assay

Figure 3: Activation induced by internal agonist ADP-ribose (1μM) and block by antagonist 2-APB (100 μM) in cells stably transfected with TRPM2.

 

TRPM2 - Manual Patch Clamp

TRPM2 assay

Figure 4: Concentration-dependent effect of 2-APB on peak currents (ITRPM2) obtained from HEK-293 cells stably transfected with TRPM2. IC50 value approximately 1.3 µM.


Back to Family
Request a Screening Quote