TRP Channels

TRPM4 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: HEK-293 / CHO

Assay Platforms: FLIPR, Manual Patch Clamp, Automated Patch Clamp

Services: HTS, single concentration / dose response studies, selectivity profiling

TRPM4 - Fluorescence Assay

trpm4 assay

Figure 1: Dose response curve of agonist ionomycin against TRPM4 using calcium sensitive dye assay.  Calculated EC50 value 6.03µM.

TRPM4 - Fluorescence Assay

trpm4 assay

Figure 2: Dose response curve of antagonist flufenamic acid against TRPM4 using calcium sensitive dye assay. Calculated IC50 value 61.8µM.

TRPM4 - QPatch Assay

trpm4 assay

Figure 3: Superimposed voltage and current traces showing inward and outward human TRPM4 current in the presence of a free internal Ca2+ concentration in combination with increasing concentrations of Ionomycin. 

TRPM4 - QPatch Assay

trpm4 assay

Figure 4: Plotted time course of free internal Ca2+ in combination with increasing concentrations of ionomycin on human TRPM4 currents. Flufenamic acid (300 µM) was used to block TRPM4 currents.


Back to Family
Request a Screening Quote