TRP Channels

TRPM5 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: CHO

Assay Platforms: Fluorescence, Manual Patch Clamp, QPatch

Services: HTS, single concentration / dose response studies, selectivity profiling

TRPM5 - Fluorescence Assay

trpm5 assay

Figure 1: Concentration-dependent activation of TRPM5 channels by ionomycin. Calculated EC50 value 4 nM.

TRPM5 - Fluorescence Assay

trpm5 assay

Figure 2: Concentration-dependent inhibition of TRPM5 channels by TPPO. Calculated IC50 value 4.79 μM.

 

TRPM5 - QPatch

trpm5 assay

Figure 3: Concentration-dependent effect of ionomycin on peak currents obtained from cells stably transfected with TRPM5.  Application of pH 6.4 fully blocked the activated currents.

 

TRPM5 - QPatch

trpm5 assay

Figure 4: Concentration-dependent effect of pH on peak currents (ITRPM5) obtained from cells stably transfected with TRPM5. IC50 value was approximately pH 6.7 .


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