TRP Channels

TRPM8 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: HEK-293

Assay Platforms: Fluorescence, Manual Patch Clamp, QPatch

Services: HTS, single concentration / dose response studies, selectivity profiling

TRPM8 - Fluorescence Assay

TRPM8 assay

Figure 1: Concentration-dependent activation of TRPM8 channels by Icillin. Calculated EC50 value 91 nM.

TRPM8 - Fluorescence Assay

TRPM8 assay

Figure 2: Concentration-dependent inhibition of TRPM8 channels by Capsazepine. Calculated IC50 value 4.88 μM.

 

TRPM8 - Manual Patch Clamp

TRPM8 assay

Figure 3: Concentration-dependent effects of Icilin on peak currents (ITRPM8) obtained from cells stably transfected with TRPM8. Calculated EC50 approximately 0.54 μM.

 

TRPM8 - Manual Patch Clamp

TRPM8 assay

Figure 4: Concentration-dependent effects of Capsazepine (in the presence of 1μM Icilin) on peak currents (ITRPM8) obtained from cells stably transfected with TRPM8. calculated IC50 approximately 50 μM.


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