TRP Channels

TRPV1 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: HEK-293

Assay Platforms: Fluorescence, Manual Patch Clamp, QPatch

Services: HTS, single concentration / dose response studies, selectivity profiling

TRPV1 - Fluorescence Assay

trpv1 assay

Figure 1: Concentration-dependent activation of TRPV1 channels by capsaicin. Calculated EC50 value 1.93 μM.

TRPV1 - Fluorescence Assay

trpv1 assay

Figure 2: Concentration-dependent inhibition of TRPV1 channels by capsazepine. Calculated IC50 value 3.89 μM.

 

TRPV1 - Manual Patch Clamp

trpv1 assay

Figure 3: Normalised concentration-response curve of Capsaicin against TRPV1 current. Calculated EC50 value 0.16 µM.

 

TRPV1 - Manual Patch Clamp

trpv1 assay

Figure 4: Normalised concentration-response curve of antagonist Capsazepine against TRPV1 currents. Calculated IC50 value 45 nM.


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