TRP Channels

TRPV2 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: HEK-293

Assay Platforms: FLIPR, Manual Patch Clamp

Services: HTS, single concentration / dose response studies, selectivity profiling

TRPV2 - Fluorescence Assay

TRPV2 assay

Figure 1: Dose response curve of agonist Cannabidiol against TRPV2 using calcium sensitive dye assay.  Calculated EC50 value 43µM.

TRPV2 - Fluorescence Assay

TRPV2 assay

Figure 2: Dose response curve of antagonist tranilast against TRPV2 using calcium sensitive dye assay. Calculated IC50 value 11.7µM.

 

TRPV2 - Manual patch clamp

TRPV2 assay

Figure 3: Dose response curve of agonist cannabidiol against TRPV2. Calculated EC50 value 7 µM.

 

TRPV2 - Manual patch clamp

TRPV2 assay

Figure 4: Inhibitory effect of antagonist Ruthenium red (RR) on cannabidiol-induced activation of TRPV2.


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