TRP Channels

TRPV3 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: HEK-293

Assay Platforms: FLIPR, Manual Patch Clamp, Automated Patch Clamp

Services: HTS, single concentration / dose response studies, selectivity profiling

TRPV3 - Fluorescence Assay

trpv3 assay

Figure 1: Dose response curve of agonist 2-APB against TRPV3 using calcium sensitive dye assay.  Calculated EC50 value 9.9µM.

TRPV3 - Fluorescence Assay

trpv3 assay

Figure 2: Dose response curve of antagonist ruthenium red against TRPV3 using calcium sensitive dye assay. Calculated IC50 value 2.5µM.

TRPV3 - QPatch

trpv3 assay

Figure 3: Concentration-dependent effect of 2-APB on normalised currents (I/Imax) obtained from HEK-293 cells expressing TRPV3. Calculated EC50 value 50 µM.

TRPV3 - QPatch

trpv3 assay

Figure 4: Concentration-dependent effect of Ruthenium red on normalised activated currents (I/Imax) obtained from HEK-293 cells expressing TRPV3. Calculated IC50 value 31µM.

 

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