TRP Channels

TRPV5 Screening Assays

Species: Human

Subunits / Accessory Proteins: N/A

Cell Type: HEK-293

Assay Platforms: FLIPR, Manual Patch Clamp, Automated Patch Clamp

Services: HTS, single concentration / dose response studies, selectivity profiling

TRPV5 - Fluorescence Assay

trpv5 assay

Figure 1: Concentration-dependent activation of TRPV5 channels by CaCl2. Calculated EC50 value 1.4 mM.

TRPV5 - Fluorescence Assay

trpv5 assay

Figure 2: Concentration-dependent inhibition of TRPV5 channels by Gd3+. Calculated IC50 value 543 μM.

 

TRPV5 - Manual Patch Clamp

trpv5 assay

Figure 3: Normalised concentration-dependent inhibitory effects of ruthenium red on currents (I/Imax) obtained from HEK-293 cells stably expressing TRPV5. Calculated IC50 value 0.15μM.

 

TRPV5 - Manual Patch Clamp

trpv5 assay

Figure 4: Normalised concentration-dependent inhibitory effects of Cobalt Chloride on currents (I/Imax) obtained from HEK-293 cells stably expressing TRPV5. Calculated IC50 value 6 μM.


Back to Family
Request a Screening Quote