Advance PAT-1 Drug Discovery with Custom Transporter Assays and High-Throughput Screening
The proton-coupled amino acid transporter PAT-1 (SLC36A1) is a critical component of neurotransmitter precursor transport and nutrient absorption. Functioning via a 1:1 proton-to-substrate exchange mechanism, PAT-1 facilitates the uptake of small neutral amino acids such as glycine and proline—essential for neurological, gastrointestinal, and metabolic health. Its involvement in diseases such as Hartnup disorder and its role in maintaining neurotransmitter balance position PAT-1 as a highly promising neurological disease drug target.
At Sygnature Discovery, we support PAT-1 drug discovery and broader transporter drug discovery through custom assay development and SSM-based electrophysiology, enabling deeper investigation into PAT-1 and other proton-coupled transporters. Our platforms deliver the flexibility and throughput you need to accelerate screening and optimize PAT-1-targeted therapies.
Discovery Our Case Study: PAT-1 Functional Characterization
In this case study, our team demonstrates the development and optimization of PAT-1 functional assays using our SURFE2R N1 and SURFE2R 96SE platforms. We generated HEK cells overexpressing PAT-1 and conducted thorough concentration-response analyses using L-Proline and L-Glycine across multiple pH conditions.
Our dual-platform approach combines flexibility of SURFE2R N1 for early assays development with the high-throughput power of the SURFE2R 96SE system- delivering scalable solutions for PAT-1 drug discovery and high-throughput transporter screening.
Supporting Your Research
At Sygnature Discovery, we deliver custom cell-based transporter assays tailored to your target biology and discovery goals.
Our capabilities include:
- Generation of overexpressing cell lines for solute carrier transporter families
- SSM-based electrophysiology for functional activity profiling
- Assay miniaturization for screening campaigns
- Expertise in amino acid transporters
- Support across lead identification and optimization workflows
